Biophysics and the Challenges of Emerging Threats
Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1 3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4 6], enzyme kinetics [7, 8], and even protein biosynthesis [...
Αποθηκεύτηκε σε:
| Κύριος συγγραφέας: | |
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| Μορφή: | Livre numérique |
| Γλώσσα: | Anglais |
| Έκδοση: |
Dordrecht :
Springer Netherlands
[20..].
Cham : Springer Nature |
| Έκδοση: | 1st ed. 2009. |
| Σειρά: | NATO Science for Peace and Security Series B: Physics and Biophysics
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| Θέματα: | |
| Διαθέσιμο Online: | Accès sur la plateforme de l'éditeur Accès sur la plateforme Istex Accès Université d'Orléans Accès INSA CVL |
| Σημείωση: |
Archives Springer e-books (Licence nationale) Archives Springer e-books (Licence nationale) |
| Autres localisations: | Voir dans le Sudoc |
| Variante du titre: | Proceedings of the NATO Advanced Study Institute on Biophysics and the Challenges of Emerging Threats Erice, Sicily, Italy 19-30 June 2007 |
| Edition sous un autre format: | • Biophysics and the challenges of emerging threats, [proceedings of the NATO advanced study institute on Biophysics and the challenges of emerging threats, Erice, Scily, Italy, 19-30 june 2007], edited by Joseph D. Puglisi, Dordrecht, Springer, 2009, 1 vol. (VII-179 p.), NATO science for peace and security series, 978-90-481-2366-7 |
| Περίληψη: | Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1 3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4 6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11 13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16 18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels |
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| Περιγραφή τεκμηρίου: | Archives Springer e-books (Licence nationale) Archives Springer e-books (Licence nationale) |
| ISBN: | 9789048123681 |
| ISSN: | 1874-6535 |
| Πρόσβαση: | Accès en ligne pour les établissements français bénéficiaires des licences nationales Accès soumis à abonnement pour tout autre établissement Conditions particulières de réutilisation pour les bénéficiaires des licences nationales. https://www.licencesnationales.fr/springer-nature-ebooks-contrat-licence-ln-2017 |