Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results
This study proposes a proteomic-based strategy for the identification of proteins and their biological origin in stucco artworks from Italian Renaissance period in the context of the ESPRIT project (Études des Stucs Polychromés de la Renaissance Italienne). Three samples were studied: Virgin and Chi...
में बचाया:
| में प्रकाशित: | URI:https://journals.openedition.org/techne, |
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| मुख्य लेखकों: | , , , , , , |
| स्वरूप: | Article ou chapitre numérique |
| भाषा: | Anglais |
| प्रकाशित: |
Technè
2021
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| विषय: | |
| ऑनलाइन पहुंच: | Accès Université d'Orléans et IFPM Accès Université d'Orléans et IFPM |
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openedition-oai:revues.org:techne_8990 |
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openedition |
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openedition-oai:revues.org:techne_8990 https://doi.org/10.4000/techne.8990 urn:doi:10.4000/techne.8990 en https://journals.openedition.org/techne/8990 2021-12-16T01:00:00Z info:eu-repo/semantics/openAccess article info:eu-repo/semantics/article urn:handle:20.500.13089/l8kj https://creativecommons.org/licenses/by-nc-nd/4.0/ Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results Technè C2RMF info:eu-repo/semantics/reference/issn/2750-6185 info:eu-repo/semantics/reference/issn/1254-7867 URI:https://journals.openedition.org/techne Aksamija, Amra Devassine, Stéphanie Bray, Fabrice Bormand, Marc Bouquillon, Anne Rolando, Christian Tokarski, Caroline protéomique stucs de la Renaissance spectrométrie de masse à haute résolution collagènes origine biologique proteomics Renaissance stucco high resolution mass spectrometry collagens biological origin This study proposes a proteomic-based strategy for the identification of proteins and their biological origin in stucco artworks from Italian Renaissance period in the context of the ESPRIT project (Études des Stucs Polychromés de la Renaissance Italienne). Three samples were studied: Virgin and Child, type Santa Maria Nuova (after Antonio Rossellino) from the Louvre Museum collection in Paris (Campana 19); Virgin and Child (after Benedetto da Maiano) (MBA 507) and Virgin and Child (Circle of Luca della Robbia) (MBA 247), both from the Museum of Fine Arts in Strasbourg collection. The analytical methodology was firstly evaluated using stucco mock up built with model proteins/binders. Various protocols were tested to extract very limited amounts of proteins from the inorganic matrix. The optimized workflow was successfully applied to historic samples showing identification of collagens and their biological origin. Cet article propose une stratégie fondée sur la protéomique pour l’identification des protéines et de leur origine biologique dans les œuvres en stuc de la Renaissance italienne, dans le cadre du programme de recherche ESPRIT (Étude des stucs polychromés de la Renaissance italienne). Les trois échantillons étudiés proviennent de La Vierge et l’Enfant, type Santa Maria Nuova, d’après Antonio Rossellino (musée du Louvre, Campana 19), une Vierge et l’Enfant d’après Benedetto da Maiano (musée des Beaux-Arts de Strasbourg, MBA 507) et une Vierge et l’Enfant de l’entourage de Luca della Robbia (musée des Beaux-Arts de Strasbourg, MBA 247). À partir d’éprouvettes de plâtre contenant différents liants protéiniques, plusieurs protocoles analytiques permettant l’extraction de très petites quantités de protéines ont été testés. Une fois optimisé, le protocole a pu être appliqué avec succès à des échantillons historiques en débouchant sur l’identification des collagènes et de leur origine biologique. |
| collection |
OpenEdition Journal |
| language |
Anglais |
| format |
Article ou chapitre numérique |
| building |
0/Bibliothèque numérique/ 1/Bibliothèque numérique/OpenEdition Journals/ |
| topic |
protéomique stucs de la Renaissance spectrométrie de masse à haute résolution collagènes origine biologique proteomics Renaissance stucco high resolution mass spectrometry collagens biological origin |
| spellingShingle |
protéomique stucs de la Renaissance spectrométrie de masse à haute résolution collagènes origine biologique proteomics Renaissance stucco high resolution mass spectrometry collagens biological origin Aksamija, Amra Devassine, Stéphanie Bray, Fabrice Bormand, Marc Bouquillon, Anne Rolando, Christian Tokarski, Caroline Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results |
| description |
This study proposes a proteomic-based strategy for the identification of proteins and their biological origin in stucco artworks from Italian Renaissance period in the context of the ESPRIT project (Études des Stucs Polychromés de la Renaissance Italienne). Three samples were studied: Virgin and Child, type Santa Maria Nuova (after Antonio Rossellino) from the Louvre Museum collection in Paris (Campana 19); Virgin and Child (after Benedetto da Maiano) (MBA 507) and Virgin and Child (Circle of Luca della Robbia) (MBA 247), both from the Museum of Fine Arts in Strasbourg collection. The analytical methodology was firstly evaluated using stucco mock up built with model proteins/binders. Various protocols were tested to extract very limited amounts of proteins from the inorganic matrix. The optimized workflow was successfully applied to historic samples showing identification of collagens and their biological origin. |
| author |
Aksamija, Amra Devassine, Stéphanie Bray, Fabrice Bormand, Marc Bouquillon, Anne Rolando, Christian Tokarski, Caroline |
| author_facet |
Aksamija, Amra Devassine, Stéphanie Bray, Fabrice Bormand, Marc Bouquillon, Anne Rolando, Christian Tokarski, Caroline |
| author_sort |
Aksamija, Amra |
| title |
Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results |
| title_short |
Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results |
| title_full |
Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results |
| title_fullStr |
Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results |
| title_full_unstemmed |
Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results |
| title_sort |
proteomic analysis of renaissance stucco: development of the analytical protocol and first results |
| publisher |
Technè |
| publishDate |
2021 |
| container_title |
URI:https://journals.openedition.org/techne |
| url |
https://ezproxy.univ-orleans.fr/login?url=https://doi.org/10.4000/techne.8990 https://ezproxy.univ-orleans.fr/login?url=https://journals.openedition.org/techne/8990 |
| _version_ |
1877293361191190528 |