Observing plant roots in their environment: current imaging options and specific contribution of two-dimensional approaches
This paper provides an overview of current options for imaging roots in their environment. Our primary aim is to describe the potential and limitations of these techniques, rather than their physical principles. We primarily discuss two-dimensional imaging techniques which, while inherently limit...
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| Materialtyp: | Article ou chapitre numérique |
| Språk: | Anglais |
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EDP Sciences
2003
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| Länkar: | Accès Université d'Orléans et IFPM Accès INSA CVL |
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edpsciences-10.1051_agro:2003019 |
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edpsciences |
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edpsciences-10.1051_agro:2003019Observing plant roots in their environment: current imaging options and specific contribution of two-dimensional approachesAlain PierretClaude DoussanEmmanuelle GarriguesJohn Mc Kirby This paper provides an overview of current options for imaging roots in their environment. Our primary aim is to describe the potential and limitations of these techniques, rather than their physical principles. We primarily discuss two-dimensional imaging techniques which, while inherently limited by their 2-dimensional nature are technically simple, cheaper, more readily accessible, and offer larger field- of-view or better resolution for a similar field-of-view than three-dimensional imaging techniques. We show that techniques involving microscopic or high-resolution X-ray imaging of roots in undisturbed soil samples have a considerable value because they can provide, at a scale which is not accessible by any other means, essential information on complex interactions between roots and soil structure. We also illustrate how 2D imaging techniques, based on either visible light or X-ray attenuation through thin growing containers (rhizotrons), can be used to study whole root systems. We conclude that, while fully appropriate 3D imaging tools will ultimately become available, in the meantime, two-dimensional techniques should be considered as relevant options for observing roots in their environment. Observation des racines dans leur environnement : techniques d'imagerie actuelles et apports spécifiques des approches 2D. Cet article offre un inventaire des techniques d'imagerie actuellement disponibles pour l'observation des racines au sein de leur environnement. Le propos est avant tout d'informer le lecteur sur le potentiel et les limites des techniques et non sur leurs principes physiques. D'autre part, l'accent est mis sur l'apport spécifique des méthodes 2D : en dépit des limitations et biais qui leur sont inhérents, ces méthodes offrent en effet, par rapport aux outils 3D actuellement disponibles, des avantages tels qu'un grand champ d'observation ou une résolution supérieure à champ d'observation équivalent, ou encore, une mise en œuvre simplifiée et un coût réduit. Ainsi, la microscopie et la radiographie X à haute résolution sont très attractives car elles permettent d'étudier, à une échelle qui demeure hors de la portée des méthodes 3D, les interactions complexes entre racines et structure du sol. On montre par ailleurs que les méthodes 2D basées sur l'atténuation de lumière visible ou de rayons X s'avèrent très utiles pour étudier le fonctionnement de systèmes racinaires entiers en rhizotrons. S'il ne fait guère de doute qu'à relativement court terme, les progrès accomplis dans le domaine de l'imagerie 3D aboutiront à la mise au point d'outils parfaitement adaptés à l'observation non destructive des racines dans leur environnement, il n'en reste pas moins que, dans l'attente de tels outils, les techniques 2D continueront de faire progresser notre connaissance des racines et de leur fonctionnement. 10.1051/agro:20030192003EDP Scienceshttps://doi.org/10.1051/agro:2003019https://www.agronomy-journal.org/10.1051/agro:2003019/pdfTexten |
| institution |
INSA |
| collection |
EDP Sciences |
| language |
Anglais |
| format |
Article ou chapitre numérique |
| building |
0/Bibliothèque numérique/ 1/Bibliothèque numérique/EDP Sciences/ |
| description |
This paper provides an overview of current options for imaging roots in their environment. Our primary aim is to describe the
potential and limitations of these techniques, rather than their physical principles. We primarily discuss two-dimensional imaging techniques
which, while inherently limited by their 2-dimensional nature are technically simple, cheaper, more readily accessible, and offer larger field-
of-view or better resolution for a similar field-of-view than three-dimensional imaging techniques. We show that techniques involving
microscopic or high-resolution X-ray imaging of roots in undisturbed soil samples have a considerable value because they can provide, at a
scale which is not accessible by any other means, essential information on complex interactions between roots and soil structure. We also
illustrate how 2D imaging techniques, based on either visible light or X-ray attenuation through thin growing containers (rhizotrons), can be
used to study whole root systems. We conclude that, while fully appropriate 3D imaging tools will ultimately become available, in the
meantime, two-dimensional techniques should be considered as relevant options for observing roots in their environment.
|
| author |
Alain Pierret Claude Doussan Emmanuelle Garrigues John Mc Kirby |
| spellingShingle |
Alain Pierret Claude Doussan Emmanuelle Garrigues John Mc Kirby Observing plant roots in their environment: current imaging options and specific contribution of two-dimensional approaches |
| author_facet |
Alain Pierret Claude Doussan Emmanuelle Garrigues John Mc Kirby |
| author_sort |
Alain Pierret |
| title |
Observing plant roots in their environment: current imaging options
and specific contribution of two-dimensional approaches |
| title_short |
Observing plant roots in their environment: current imaging options
and specific contribution of two-dimensional approaches |
| title_full |
Observing plant roots in their environment: current imaging options
and specific contribution of two-dimensional approaches |
| title_fullStr |
Observing plant roots in their environment: current imaging options
and specific contribution of two-dimensional approaches |
| title_full_unstemmed |
Observing plant roots in their environment: current imaging options
and specific contribution of two-dimensional approaches |
| title_sort |
observing plant roots in their environment: current imaging options
and specific contribution of two-dimensional approaches |
| publisher |
EDP Sciences |
| publishDate |
2003 |
| url |
https://ezproxy.univ-orleans.fr/login?url=https://doi.org/10.1051/agro:2003019 https://ezproxy.insa-cvl.fr/login?url=https://doi.org/10.1051/agro:2003019 |
| _version_ |
1877237856669270016 |