Cell Separation : Fundamentals, Analytical and Preparative Methods

Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various biolog...

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Detalhes bibliográficos
Autor principal: Kumar, Ashok
Outros Autores: Galaev, Igor Yu (Éditeur intellectuel), Mattiasson, Bo, 1945- (Éditeur intellectuel)
Formato: Livre numérique
Idioma:Anglais
Publicado em: Berlin, Heidelberg : Springer Berlin Heidelberg [20..].
Cham : Springer Nature
Edição:1st ed. 2007.
Colecção:Advances in Biochemical Engineering/Biotechnology 106
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Nota: Archives Springer e-books (Licence nationale)
Archives Springer e-books (Licence nationale)
Autres localisations: Voir dans le Sudoc
Variante du titre:With contributions by numerous experts
Edition sous un autre format:• Cell separation, fundamentals, analytical and preparative methods, Ashok Kumar, Igor Yu Galaev, Bo Mattiasson, Berlin, Springer, 2007, 1 vol. (XII-203 p.), Advances in biochemical engineering / biotechnology, 978-3-540-75262-2
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505 1 |a Methods in Cell Separations Flow Cytometry and Cell Sorting Cell Isolation and Expansion Using Dynabeads ® Affinity Adsorption of Cells to Surfaces and Strategies for Cell Detachment Chromatography of Living Cells Using Supermacroporous Hydrogels, Cryogels Hollow-Fibre Affinity Cell Separation Cell Partitioning in Aqueous Two-Phase Polymer Systems Development of Separation Technique for Stem Cells 
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520 |a Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various biological applications has increased. Ef?cient and cost-effective techniques for fr- tionation and isolation of target cell types are necessary to provide pure cell populations for diagnostics, biotechnological and biomedical applications. One can see a considerable need, both in biomedical research and in di- nostic medicine, for the speci?c separation of a discrete population of cells from a mixture. For example, in the ?eld of tissue engineering, isolation of stemcellsfromtissuesororgansisofparticularlygreatimportance.Moreover, understanding cell developmental pathways becomes increasingly signi?cant as diagnosis and treatment of diseases turns more to the molecular level. The diagnosis of cell-related diseases requires methods of detection, isolation and theanalysisofindividualcells,regardlessoftheirrelativecontentinthetissue. Since cell-based therapies now turn towards more realistic medical options, developing an effective separation system for large-scale cell separation has becomechallengingresearchgoalforcellbiologistsandbiotechnologists.The ideal technique should provide in a short time a good yield of cells with high puritywhile maintaining cellfunction.Despite the growingneed formethods to separate cells into cell subpopulations, the existing cell-separation te- niques stillhave somelimitations when the desired degree ofperformance on apreparativescaleisrequired.Wewillseemoreresearchfocusinthisdirection in the future. The traditional techniques of micro?ltration, ultra?ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci?cityandproblemswithscalingup 
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650 |a Chimie 
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650 |a Chimie analytique 
700 1 |a Galaev, Igor Yu.  |4 edt 
700 1 |a Mattiasson, Bo,  |d 1945-  |4 edt 
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