Culture of animal cells : a manual of basic technique
Salvato in:
| Autore principale: | |
|---|---|
| Natura: | Livre papier |
| Lingua: | Anglais |
| Pubblicazione: |
New York :
Wiley-Liss
cop. 1994.
|
| Edizione: | 3rd ed. |
| Soggetti: | |
| Autres localisations: | Voir dans le Sudoc |
Sommario:
- Biology of the cultured cell
- Design and layout of the laboratory
- Equipping the laboratory
- Aseptic technique
- Laboratory safety and biohazards
- The culture environment : substrate, gas phase, medium and temperature
- Preparation and sterilization
- Disaggregation of the tissue and primary culture
- Maintenance of the culture : cell lines
- Cloning and selection of specific cell types
- Physical methods of cell separation
- Induction of differentiation
- The transformed phenotype
- Measurement of viability and cytotoxicity
- Culture of specific cell types
- Culture of tumor tissue
- Three-dimensional culture systems
- Specialized techniques