Drosophila : methods and protocols
Salvato in:
| Altri autori: | |
|---|---|
| Natura: | Livre papier |
| Lingua: | Anglais |
| Pubblicazione: |
Totowa, N.J. :
Humana Press
cop. 2008.
|
| Serie: | Methods in molecular biology
420 |
| Soggetti: | |
| Autres localisations: | Voir dans le Sudoc |
Sommario:
- Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand P-element mutagenesis / Thomas Hummel and Christian Klämbt EMS screens : from mutagenesis to screening and mapping / Christian Bökel RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre Immunolabeling of embryos / H.-Arno J. Müller Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist Immunolabeling of imaginal discs / Thomas Klein Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.] Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol Extraction and immunoblotting of proteins from embryos / Andreas Wodarz Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder