Molecular cloning : a laboratory manual
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| Auteurs principaux: | , |
|---|---|
| Formato: | Livre papier |
| Idioma: | Anglais |
| Publicado em: |
Cold Spring Harbor :
Cold Spring Harbor Laboratory Press
cop. 2001.
|
| Edição: | 3rd edition. |
| Assuntos: | |
| Autres localisations: | Voir dans le Sudoc |
Sumário:
- Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning Ch. 2. Bacteriophage and its vectors Ch. 3. Working with bacteriophage M13 vectors Ch. 4. Working with high-capacity vectors Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis Ch. 6. Preparation and analysis of eukaryotic genomic DNA Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction Ch. 9. Preparation of radiolabeled DNA and RNA probes Ch. 10. Working with synthetic oligonucleotide probes Ch. 11. Preparation of cDNA libraries and gene identification Ch. 12. DNA sequencing Ch. 13. Mutagenesis Ch. 14. Screening expression libraries Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli Ch. 16. Introducing cloned genes into cultured mammalian cells Ch. 17. Analysis of gene expression in cultured mammalian cells Ch. 18. Protein interaction technologies